Structural Analysis of Supramolecular Assemblies by Hybrid Methods

In order to understand the workings of a living cell, we need to know the structures and dynamics of individual biological macromolecules, their complexes and networks, organelles, and whole cells, thus interfacing structural biology with cell biology, systems biology, proteomics, chemical biology, and biophysics. The accuracy, precision, completeness, and efficiency of structural characterization are maximized by hybrid or integrative approaches that combine data from multiple types of experimental methods through the use of computational analysis and modeling methods. These experimental methods include X-ray crystallography, NMR spectroscopy, electron microscopy, small angle X-ray scattering, mass spectrometry, chemical cross-linking, super-high resolution optical microscopy, optical microscopy, and others. The goal of this meeting is to highlight the strengths and limitations of the current hybrid approaches as well as to identify new promising advances in experimental methods for collecting the data and computational methods for converting the data to models. Finally, hybrid approaches will be demonstrated by their applications to important and challenging biological problems, spanning a wide range of length and time scales, from atomic structures of individual proteins to low-resolution representations of whole cells.
+ show speakers and program
SUNDAY, MARCH 3

16:00—20:00
Arrival and Registration

Pre Function

MONDAY, MARCH 4

07:00—08:00
Breakfast

Granhall
08:00—09:00
Welcome and Keynote Address
Registered attendees can view abstracts starting on 02/03/2013

Mountain/Lake
Speaker to be Announced

09:00—12:00
Computation for Hybrid Approaches
Registered attendees can view abstracts starting on 02/03/2013

NOTE: This session will introduce major challenges in structural modeling of biological systems at different resolutions, from atomic to cellular, based on varied experimental datasets. Such challenges include representing the system, converting data into scoring functions, finding good scoring solutions, and analysing the results.

Mountain/Lake
Andrej Sali, University of California, San Francisco, USA
Integrative Structure Determination of Macromolecular Assemblies

David Baker, University of Washington, USA
Hybrid Methods for Atomic Structure Determination

Gregory A. Voth, University of Chicago, USA
Multi-Scale Models of Biological Macromolecules

Zaida (Zan) Luthey-Schulten, University of Illinois at Urbana-Champaign, USA
Whole Cell Simulations

Short Talk Chosen from Abstracts

09:40—10:00
Coffee Break

Pre Function
12:00—13:00
Poster Setup

Bay
13:00—22:00
Poster Viewing

Bay

On Own for Lunch and Recreation

16:30—17:00
Coffee Available

Pre Function
17:00—19:00
Hybrid Approaches to Studying Dynamic Systems
Registered attendees can view abstracts starting on 02/03/2013

NOTE: This session will highlight successes and challenges in using hybrid methods to describe the dynamics of individual macromolecules and their assemblies. Presentations will motivate further method development by the needs of structural biologists interested in specific systems.

Mountain/Lake
James R. Williamson, The Scripps Research Institute, USA
Hybrid Studies of Ribosome Assembly in Cells

Joseph D. Puglisi, Stanford University, USA
Hybrid Studies of the Ribosome Function

Klaus Schulten, University of Illinois at Urbana-Champaign, USA
Simulation of Dynamics of Large Macromolecular Systems

Short Talk Chosen from Abstracts

19:00—20:00
Dinner

Granhall
19:30—22:00
Poster Session 1

Bay
20:00—21:00
Social Hour

Bay

TUESDAY, MARCH 5

07:00—08:00
Breakfast

Granhall
08:00—11:00
Hybrid Approaches to Studying Macromolecular Structures
Registered attendees can view abstracts starting on 02/03/2013

NOTE: This session will highlight successes and challenges in using hybrid methods to describe the structures of individual macromolecules and their assemblies. Presentations will motivate further method development by the needs of structural biologists interested in specific systems.

Mountain/Lake
Ian A. Wilson, The Scripps Research Institute, USA
Hybrid Approaches to Antibody - Virus Systems

Holger Stark, Max Planck Institute for Biophysical Chemistry, Germany
Hybrid Structural Studies of the Splicing Machinery

Wolfgang P. Baumeister, Max Planck Institute of Biochemistry, Germany
Hybrid Studies of the Structure and Function of the 26S Proteasome

David A. Agard, University of California, San Francisco, USA
Hybrid Approaches to Tubulin Assembly

Short Talk(s) Chosen from Abstracts

09:20—09:40
Coffee Break

Pre Function
11:00—13:00
Poster Setup

Bay
13:00—22:00
Poster Viewing

Bay

On Own for Lunch and Recreation

14:30—16:30
Workshop and Panel 1: Critical Issues for Computing, Analyzing, Visualizing, Archiving, and Disseminating Hybrid Models

Short Talks Chosen from Abstracts

16:30—17:00
Coffee Available

Pre Function
17:00—19:00
Hybrid Approaches to Studying Cellular Organization
Registered attendees can view abstracts starting on 02/03/2013

NOTE: This session will highlight approaches, tools, successes and challenges in using hybrid methods to describe the organization of whole cells.

Mountain/Lake
Benjamin Geiger, Weizmann Institute of Science, Israel
Talk Title to be Determined

Frank Alber, University of Southern California, USA
Genome Organization by Integrative Structure Determination

Graham Johnson, University of California, San Francisco, USA
Modeling and Animating the Cellular Mesoscale

Short Talk Chosen from Abstracts

19:00—20:00
Dinner

Granhall
19:30—22:00
Poster Session 2

Bay
20:00—21:00
Social Hour

Bay

WEDNESDAY, MARCH 6

07:00—08:00
Breakfast

Granhall
08:00—11:00
Single Molecule Methods
Registered attendees can view abstracts starting on 02/03/2013

NOTE: This session will focus on computational and experimental methods for studying single molecules, to localize them in the cell, position them with respect to other structures in the cell, and in particular to describe their dynamics. The methods will be illustrated by their application to specific biological systems.

Mountain/Lake
Stefan W. Hell, Max Planck Institute for Biophysical Chemistry, Germany
Nanoscopy with Focused Light

Jennifer A. Lippincott-Schwartz, NICHD, National Institutes of Health, USA
Use of Photoactivated Localization Microscopy (PALM) for High-Density Mapping of Single Molecules and their Trajectories at the Nanoscale

Scott C. Blanchard, Weill Cornell Medical College, USA
Single-Molecule Imaging of Biological Systems

X. Sunney Xie, Harvard University, USA
Single Molecule Enzymology

Short Talk(s) Chosen from Abstracts

09:20—09:40
Coffee Break

Pre Function

On Own for Lunch and Recreation

14:30—16:30
Workshop and Panel 2: Latest Advances in Hybrid Methods

NOTE: This workshop will precede the previous session with the same title. It will feature speakers selected from the abstract submissions as well as a panel-led discussion of the most timely issues in integrative characterization of the structures and dynamics of macromolecular systems.

Short Talks Chosen from Abstracts

16:30—17:00
Coffee Available

Pre Function
17:00—19:00
Latest Advances in Hybrid Methods
Registered attendees can view abstracts starting on 02/03/2013

NOTE: This session will present recent methodological advances in hybrid approaches as well as in individual methods for structure and dynamics characterization that can be incorporated into hybrid approaches.

Mountain/Lake
Brian T. Chait, Rockefeller University, USA
Mass Spectrometry and Proteomics for Hybrid Structure Determination

Carolyn A. Larabell, University of California, San Francisco, USA
X-Ray Tomography of Cells

Michael Nilges, Institut Pasteur, France
Inferential Structure Determination

Short Talk Chosen from Abstracts

19:00—20:00
Dinner

Granhall
19:00—20:00
Social Hour

Granhall
20:00—23:00
Entertainment

Granhall

THURSDAY, MARCH 7


Departure


*Session Chair †Speaker invited, not yet responded.

3 Mar - 7 Mar 2013
Tahoe City
United States of America
meeting website