Fundamentals and Applications of Fluorescence Microscopy in Modern Cell Biology

An intensive, one week course which is organised by "American Society for Cell Biology" and "Koc University"

The participants will gain theoretical and practical experience related with fluoresence and confocal microscopy. The course will provide hands on experience with advanced imaging techniques. All attendees will use fully equipped research laboratories with the aid of expert instructors. There will be many opportunities to work with different types of microscopes. Attendees should leave the course comfortable with both principals and practice related with fluorescence and confocal imaging.
+ show speakers and program
Dean of Medicine at Koc
Jim Spudich ASCB-IAC, Stanford University, USA
Organization remarks and course overview:

Ranan Aktas, Koc University, Turkey
Mahasin Osman, Brown University, USA
Didactic Lecture: Microscopy in modern cell biology: General overview, Mahasin Osman, Brown University, USA
10:15-10:30 am Break
10:30-11:30 am Didactic Lecture: The optical microscope: A primer on the photophysics of fluorescence emmission (quantum yield, lifetime, polarization, energy) and their application to quantitative fluorescence microscopy. Gerard Marriot, University of California, Berkeley, USA
11:30-12:30 am Didactic Lecture: Fundamentals of Fluorescence Microscopy: Microscope parts, lamps and alignment –Ranan Gulhan Aktas, Koc University, Istanbul, TURKEY
12:30-1:30 pm Lunch
1:30-2:30 pm
Laboratory: Identify microscope parts, light path, alignment, resolution etc. Zeiss, Leica, Olympus, Nikon and faculty

Lab leaders: Alp Can, Robert H. Chow, Oktay Gundogdu, Hasan Eser, Burak Buyrukbilen
Instructors: All staff
2:30-3:15 pm Laboratory: Fluorescence Microscopes, ZEISS
3:15-3:30 pm Coffee Break
3:30-4:30 pm Didactic Lecture: Instrumentations, fundamentals and Functional Approaches in Confocal Microscopy. Alp Can, Ankara University, Ankara, Turkey
4:30-5:30 pm Laboratory: Introduction to Confocal Microscopy. NIKON
5:45-7:00 pm Discussion session, Q&A and evaluation
7:00-8:00 pm Dinner
8:15-9:45 pm Research Talk (Application): Jim Spudich: Myosin motors – from single molecule motility assays to cardiac disease. Stanford University, USA
9:45-10:30 pm Free time on Microscopes with Zeiss, Leica, Olympus, Nikon and faculty


Monday, August 13
FLUORESCENCE MICROSCOPY
9:00-10:30 am Didactic Lecture: Fluorescent indicators: How to choose the right fluorescent proteins, Fluorophores (CFP, GFP, YFP, dsRED, mCherry) for your application: Sub-cellular localization using fluorescent proteins. Batu Erman, Sabanci University, Turkey & Sercin Karahuseyinoglu, Suleymaniye Research & Training Hospital, Turkey
10:30-10:45 am Coffee break
10:45-12:30 pm Didactic Lecture: Acquiring high-quality, quantitative images, resolution, signal-to-noise, and objective lens selection. Robert H. Chow, University of Southern California, USA
12:30-1:30 pm Didactic Lecture: Photonics: Optical detectors, Digital image acquisition and how to choose the right camera. Gerard Marriott, University of California, Berkeley, USA
1:30-2:30 pm Lunch
2:30-5:30 pm Laboratory: Fluorescence lab. Acquire images from fixed cells, apply the principles of signal-to-noise. Perform simple vital staining, time-lapse imaging, save your images for image analysis. Lab leader: Sercin Karahuseyinoglu & Deniz Yucel
5:30-6:30 pm Discussion session, Q&A and evaluation
7:00-8:00 pm Dinner
8:15- 9:00 pm Developing Technologies in Microscopy, LEICA
9:15-10:00 pm Free time on the microscopes with venders

Tuesday, August 14
FLUORESCENCE MICROSCOPY
9:00-10:30 am Lecture: Single and Multi-photon Fluorescence Microscopy Imaging: Principles and Application. Yusuf Tan, Neurobiology and Biophysics, Bogazici University, Turkey
10:30-10:45 am Coffee break
10:45-11:45 am Didactic Lecture: FRET imaging and its various applications. Halil Bayraktar, Chemistry, Koc University, Turkey
11:45-12:45 pm Didactic Lecture: New Technology in Fluorescence Microscopy: STED, FPALM, STORM and Structured illumination, Robert H. Chow, University of Southern California, USA
1:00-2:00 pm Lunch
2:00-3:00 pm Didactic Lecture: Practical Applications of Laser Scanning Confocal Microscopy. Deniz Yucel, Acibadem University, Turkey
3:00-4:00 pm Didactic Lecture: New imaging approaches, cell cultures to creatures: High-resolution ultrasound, and noninvasive optical imaging strategies. Mahasin Osman, Brown University, USA
4:00-4:15 pm Coffee Break
4:15-6:30 pm Journal Club: Research paper demonstrating
7:00-8:00 pm Dinner
8:15-10:00 pm Research Talk: Our newest developments in single molecule fluorescence imaging. Jim Spudich, Stanford University, USA

Wednesday, August 15
IMAGE ANALYSIS WORKSHOP
QUANTITATIVE IMAGE ANALYSIS AND VISUALIZATION

9:00-10:30 am Didactic Lecture: The application of small probes for optical manipulation of biological systems: from caged probes to optical switches and photo switchable genetically encoded proteins. Gerard Marriott, University of California, Berkeley, USA
10:30-10:45 am Coffee break
10:45-12:30 pm Didactic Lecture: 3D and 4D Image Visualization and Analysis: Delissa Garcia, IMARIS, England
12:30-1:30 pm Lunch
1:30- 4:30 pm Laboratory: Image analysis Leaders: Gerard Marriott and Brett Hoover
4:30-4:45 pm Coffee break
4:45-6:00 pm Video presentation: Bitplane Scientific Software
6:00-7:00 pm Research Talk: Visualizing prostate cancer androgen receptor signalling in vivo: Nathan Lack, School of Medicine, Koc University
7:00-8:00 pm Dinner
8:15-9:45 pm Research Talk: "Optical switches and molecular actuators: New tools for the imaging and manipulation of proteins in living systems" Gerard Marriott, Berkeley University, USA
9:45-11:00 pm Free time for interaction with venders

Thursday, August 16
TOTAL INTERNAL REFLECTION WORKSHOP:
PRINCIPLES AND DEMONSTRATION: OLYMPUS 4-LINE TIRF MICROSCOPE

9:00-10:30 am Didactic Lecture: Nonlinear optical microscopy: TIRF microscopy, Robert H. Chow, University of Southern California, USA
10:30-10:45 am Coffee break
10:45-12:30 pm Didactic Lecture: TIRF Microscope Features, OLYMPUS
12:30-1:30 pm Lunch
1:30- 4:30 pm Laboratory: Practice on TIRF, Alignment, Instrumentation and Imaging
4:30-4:45 pm Coffee break
4:45-5:15 pm From Fluorescence Microscope to Electron Microscope: Preparation of Specimens for Electron Microscopy, Leica, Andreas Nowak, Vienna, Austuria
5:30-7:00 pm Round Table: Students Preparation for Presentation, Q&A
7:00-8:00 pm Dinner
8:15-9:45 pm Research Talk: Application of TIRF in Insulin Secretion and Diabetes, Robert H. Chow, University of Southern California, USA
9:45-11:00 pm Free time with venders and faculty on TIRF

11 Aug - 17 Aug 2012
Istanbul
Turkey
meeting website