Targeted Genome Editing Using Zinc Finger Nucleases

Zinc finger nucleases (ZFNs) enable manipulation of the genome with unprecedented ease and precision. ZFNs are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks (DSBs) in DNA at user-specified locations. DSBs are important for site-specific mutagenesis in that they stimulate the cell's natural DNA-repair processes, namely homologous recombination and non-homologous end joining. Using well-established and robust protocols, these cellular processes can be harnessed to generate precisely targeted genomic edits resulting in cell lines, including somatic cell lines, with targeted gene deletions (knockouts), integrations, or modifications. Featured lectures will provide in-depth discussion on the fundamentals of ZFNs as well as cutting edge applications for targeted gene knockout, integration and tagging. Additionally, each of the participants will conduct laboratory exercises to become proficient in various techniques for ZFN delivery, identification of mutants, and methods for quantitating ZFN efficiency. Registrants should be familiar with basic mammalian cell culture techniques, PCR and cloning.
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Scientific Organisers

Vladimir Benes, EMBL Heidelberg, Germany

Jacqueline Dreyer, EMBL Heidelberg, Germany

17 Sep - 19 Sep 2012

Heidelberg
Germany
meeting website