Next Generation Sequencing

Next-generation sequencing has become the premier tool in genetic and genomic analysis. This laboratory-based course is directed at scientists who will be generating and interpreting sequence information in their research and wish to gain a better understanding of the techniques involved and their applications. This course is not meant to replace the manufacturers’ training normally supplied with new instruments, nor is it intended to be a training course for those solely interested in next-generation sequencing bioinformatics.

The course will include theoretical and practical information on all of the next-generation sequencing systems available and those on the near horizon. The laboratory element will cover a mix of technologies that will be determined nearer the time, but will be chosen from platforms like the Ion Torrent, PacBio and Illumina Miseq / HiSeq. One aim of the course is to allow participants to make informed decisions about which technology to apply to solve specific research questions they may face in the future. A variety of applications will be covered as time permits, e.g., RNA-Seq, target enrichment, bacterial sequencing, cancer genomics, human variation analysis, etc. All the basic techniques of post-sequencing analysis will be covered, QC, alignment, assembly, variant calling, etc.

Programme

Library Prep
• Making libraries from samples
• Both theory and practical sessions

Sequencing
• Overview: Motivation and fundamental concepts
• Detailed description of prominent sequencing technologies: current and future (3rd generation) platforms
• Hands-on laboratory work: preparing chips for sequencing and performing sequencing runs on various platforms

Data analysis
• Data QC: How to determine if a run has performed well
• Alignment to a reference, de-novo assembly, SNP and structural variant-calling of next generation DNA and RNA data
• IT and the data storage problem
+ show speakers and program
Course instructors

Harold Swerdlow (Head of Sequencing Technology, The Wellcome Trust Sanger Institute, Cambridge, UK)
Carol Churcher (Head of Sequencing Operations, The Wellcome Trust Sanger Institute, Cambridge, UK)
Tony Cox (Head of Sequencing Informatics, The Wellcome Trust Sanger Institute, Cambridge, UK)
2011 Guest speakers (2012 Guest speakers TBC shortly)

Julian Parkhill (Wellcome Trust Sanger Institute, UK)
Richard Durbin (Wellcome Trust Sanger Institute, UK)
Duncan Odom (Wellcome Trust Sanger Institute, UK)
Nick Thomson (Wellcome Trust Sanger Institute, UK)
Susanna Cooke (Wellcome Trust Sanger Institute, UK)

8 Oct - 16 Oct 2012
cambridge
United Kingdom
meeting website