Protein Interactions and Networks

Course summary

This popular lab-based course provides training in the latest laboratory techniques and bioinformatics tools necessary to discover protein interactions and understand protein function.

The week-long course will combine practical work with computer-based data analysis. The practical programme will be complemented by specific lectures in topical areas as well as a panel of distinguished guest speakers, who will present the latest research in the field.
Programme

Protein Complexes by Mass Spectrometry

affinity purification using TAP tag approach
protein Identification by Mass Spectrometry.

Yeast Two-Hybrid

rapid methods for making Bait and Prey clones
using mating assays to investigate specific interactions
library screening: faster ways to find new partners.

Binary Protein Interactions/BIAcore

low affinity extracellular receptor-ligand interactions
biophysical protein interaction parameters (binding constants and kinetics).

Bioinformatics

network and pathway visualisation
protein interaction prediction and analysis
data integration.
+ show speakers and program
Course instructors

Jyoti Choudhary (Wellcome Trust Sanger Institute, Hinxton, UK)
Chris Sanderson (University of Liverpool, UK)
Gavin Wright (Wellcome Trust Sanger Institute, Hinxton, UK)
Anton Enright (European Bioinformatics Institute, Hinxton, UK)
2012 Guest speakers to include:

Ulf Landegren (Uppsala University, Sweden)
Matthias Gstaiger (ETH Zurich, Switzerland)
Alex Bateman (Wellcome Trust Sanger Institute, Hinxton, UK)

19 Aug - 25 Aug 2012
cambridge
United Kingdom
meeting website