Workshop on Techniques in Modeling Human Cancer in Mice

Cancer is a genetically complex and biologically heterogeneous group of disorders. It has become increasingly clear that the laboratory mouse, the best genetically defined experimental model organism for humans, presents a major opportunity for rapid advancement in understanding the genetic basis and underlying biology of cancer. The overall goal for our course, “Workshop on Techniques in Modeling Human Cancer in Mice”, is to train a small group of young scientists (predoctoral, postdoctoral trainees, new investigators) in the use of genetically defined laboratory mice as genetic tools for asking questions about gene function and the role of genetics in the biology of cancer. Students completing the course will acquire a practical knowledge of how to characterize and analyze specific mouse cancer models. The models chosen will reflect several organ sites including colon, prostate, mammary, lung, brain and blood. The course will consist of didactic lectures in the morning to introduce particular organ sites and the characteristics of cancers associated with those organ sites, followed by intensive laboratory sessions whereby students will gain hands on experience in the manipulation and analysis of relevant mouse models. Sessions will include fixed and live cell imaging techniques, cytometry, surgical approaches to the implantation of tumors, and pathology.

These Aims will be accomplished by offering an intensive 10-day course to 16 young investigators chosen for their outstanding research potential. They will interact with a group of prominent mouse geneticists and cancer biologists both from The Jackson Laboratory and other prominent institutions. The size of the class will be kept deliberately small in order to achieve a desirable level of student-faculty interaction and to permit extensive laboratory training and practice for the students. The course will be held annually during the month of October at Highseas, The Jackson Laboratory’s residential oceanfront conference facility. Lectures, discussions, workshops, and demonstrations will be held morning, afternoon, and evening for a total of approximately 72 hours of didactic and hands-on training. We are asking for a full five years of support in this application.
+ show speakers and program
Workshop on Techniques in Modeling Human Colon Cancer in Rodents
October 12-16, 2010
Contact: Erin McDevitt – Events and Meetings Planner
Email: erin.mcdevitt@jax.org
Tuesday, October 12
th

Location: Highseas Conference Center
4:30PM Registration and social hour
5:30 Dinner
Location: Highseas Conference Center – Living Room
6:30 Orientation
John Macauley, Ph.D., The Jackson Laboratory
Current and Future Challenges in Colon Cancer Research
James Amos-Landgraf, Ph.D. & Richard Halberg, Ph.D., University of Wisconsin and David
Threadgill, Ph.D., North Carolina State University
Wednesday, October 13
th

Location: Highseas Conference Center

7:00AM Breakfast
Location: Highseas Conference Center – 3
rd
Floor Conference Room
8:30 Introduction to intestinal biology
Robert Cormier, Ph.D., University of Minnesota
9:00 Introduction to the Min mouse
Richard Halberg, Ph.D., University of Wisconsin 9:15 Introduction to the PIRC rat
Jim Amos-Landgraf, Ph.D., University of Wisconsin
9:30 Animal models of inflammatory bowel disease
Amy Irving, B.S., University of Wisconsin
10:00 Van transports participants to GRB Training Lab
Location: GRB Training Laboratory
10:15 Working with mice and rats
James Amos-Landgraf, Ph.D., Jamie Hadac, B.S., Richard Halberg, Ph.D. and Amy Irving,
B.S., University of Wisconsin
Location: Roscoe’s Cafeteria
12:30PM Lunch
Location: GRB Training Laboratory
1:30 Endoscopy of mice and rats
James Amos-Landgraf, Ph.D., Jamie Hadac, B.S., Richard Halberg, Ph.D. and Amy Irving,
B.S., University of Wisconsin
Florescent endoscopy
VisualSonics

5:00 Discussion – Animal models of colon cancer
6:00 Vans transport Participants to Highseas Conference Center
Location: Highseas Conference Center
6:30 Dinner
Location: Highseas Conference Center – 3
rd
Floor Conference Room
7:30 Genetic analysis of cancer
David Threadgill, Ph.D., North Carolina State University
Discovery and Analysis of Mom1
Robert Cormier, Ph.D., University of Minnesota Thursday, October 14
th
5:45AM Optional Hike: Precipice Trail, One of the steepest and most dramatic climbs in Acadia,
the hiking trail has many rungs and ladders along the trail’s length and is approximately
1.6 mile/2.6 kilometers round trip providing you with a bird’s-eye view of the coast.
Location: Highseas Conference Center
7:00 Breakfast

Location: Highseas Conference Center – 3
rd
Floor Conference Room
8:30 Modeling advanced cancers
David Threadgill, Ph.D., North Carolina State University
10:00 Van transports participants to GRB Training Lab
Location: GRB Training Lab
10:15 Techniques for modeling metastasis
David Threadgill, Ph.D. and Rachel Lynch, Ph.D., North Carolina State University
Location: Roscoe’s Cafeteria
12:30PM Lunch
Location: GRB Training Lab
1:30 Gastrointestinal dissections and scoring
James Amos-Landgraf, Ph.D., Jamie Hadac, B.S., Richard Halberg, Ph.D. and Amy Irving,
B.S., University of Wisconsin
6:00 Vans transport Participants to Highseas Conference Center
Location: Highseas Conference Center
6:30 Dinner
Friday, October 15
th

Location: Highseas Conference Center
7:00AM Breakfast
Location: Highseas Conference Center – 3
rd
Floor Conference Room 8:30 New genetic tools in rodents
James Amos-Landgraf, Ph.D., University of Wisconsin
9:15 Genetic screens with the Sleeping Beauty transposon
Robert Cormier, Ph.D., University of Minnesota
10:00 Van transports participants to GRB Training Lab
Location: GRB Training Lab
10:15 Crypt and colonocyte isolation
Rachel Lynch, Ph.D., North Carolina State University
Tumor scoring, continued
James Amos-Landgraf, Ph.D., Jamie Hadac, B.S., Richard Halberg, Ph.D. and Amy Irving,
B.S., University of Wisconsin
Location: Roscoe’s Cafeteria
12:30PM Lunch
Location: GRB Training Lab
1:30 Crypt isolation- continued
Rachel Lynch, Ph.D., North Carolina State University
Beta-catenin IHC
James Amos-Landgraf, Ph.D, and Amy Irving, B.S., University of Wisconsin
6:00 Vans transport Participants to Highseas Conference Center
Location: Highseas Conference Center
6:30 Dinner
7:30 Perspective gained and needed
William Dove, Ph.D., University of Wisconsin - discussion leader
Saturday, October 16, 2010
Location: Highseas Conference Center
7:00AM Breakfast
Location: Highseas Conference Center – 3
rd
Floor Conference Room
8:30 Comparative Pathology – veterinary perspective
Ruth Sullivan, Ph.D., University of Wisconsin Comparative Pathology – clinical perspective
Kay Washington, M.D., Vanderbilt University
10:00 Van transports participants to GRB Training Lab
Location: GRB Training Lab
10:15 IHC - continued
James Amos-Landgraf, Ph.D, and Amy Irving, B.S., University of Wisconsin
Pathology – continued
Ruth Sullivan, Ph.D., University of Wisconsin and Kay Washington, M.D., Vanderbilt
University
Location: Roscoe’s Cafeteria
12:30PM Lunch
Location: GRB Training Lab
1:30 Clean up, wrap up discussion
2:30 Vans transport Participants back to Highseas Conference Center
Location: Highseas Conference Center
6:00 Dinner

11 Oct - 22 Oct 2012

Bar Harbor
United States of America
meeting website