Workshop on Phenotyping Mouse Models of Human Lung Disease

This hands-on workshop will cover lung mechanics and techniques for measuring function in mice and how mouse models may be used to study human pulmonary biology and disease. After an initial day of basic mouse genetics, nomenclature, caging and breeding strategies, anesthesia and surgical techniques, the workshop quickly focuses on models of lung disease, pathology, lung mechanics and imaging. Laboratory sessions covering airway responsiveness, airway hyper-responsiveness (AHR), lung volumes and PV curves, morphometry, sampling techniques and mouse lung pathology will be held in the afternoons.
+ show speakers and program
Rev. 9/13/11 FINAL
Phenotyping Mouse Models of Human Lung Disease
September 18 – 23, 2011
WORKSHOP SCHEDULE
This schedule and is subject to change.
Unless noted otherwise, all sessions are in the
Highseas Conference Room or GRB Training Laboratory
For more information, please contact Nancy.Place@jax.org
Sunday, September, 18
Arrival at Highseas Conference Center anytime after 12:30 PM for registration/check in.
4:00 PM Reception and Registration at Highseas
6:00 PM Dinner at Highseas
Monday, September 19 - The Mouse and How We Use It
8:10 AM Introduction and Orientation
8:30 AM Mouse Models of Lung Disease
Wayne Mitzner, Ph.D., Johns Hopkins Bloomberg School of Public Health
9:15 AM History, Genetics, and Nomenclature
John Macauley, Ph.D., The Jackson Laboratory
10:00 AM Husbandry, Caging and Breeding
Dorcas Corrow, B.S., L.A.T.G., The Jackson Laboratory (retired)
10:45 AM Break
11:00 AM Anesthesia of the Mouse
Anthony Nicholson, B.V.Sc., , Ph.D.Rev. 9/13/11 FINAL
12:00 PM Lunch, Highseas Dining Room
1:00 PM Transportation to GRB Training Lab
1:15 PM Lab: Biomethods #1
Kristen Cough, B.S., The Jackson Laboratory
2:15 PM Break
2:30 PM Lab: Biomethods #2 (including an introduction to the flexiVent)
Annette Robichaud, Ph.D., SCIREQ Scientific Respiratory Equipment Inc.
4:30 PM Lab Clean Up
4:45 PM Transportation to Highseas
6:00 PM Dinner, Highseas Dining Room
7:00 – 8:30 “Roundtable Discussion”
Highseas Living Room
Tuesday, September 20 - Assessment of Mouse Lung Function
8:00 AM Foundations of Lung Function
Jason Bates, Ph.D., The University of Vermont
8:45 AM Lung Mechanics/Forced Oscillation
Jason Bates, Ph.D., The University of Vermont
9:30 AM Break
9:45 AM Imaging the Lung
Eric Hoffman, Ph.D., University of Iowa
10:45 AM Lung Volumes and Pressure-Volume Curves
Wayne Mitzner, Ph.D., Johns Hopkins Bloomberg School of Public Health
11:30 AM Lunch, Highseas Dining Room
12:30 PM Transportation to GRB Training Lab
1:00 PM Concurrent, rotating labs:
Lung Mechanics and Airway Responsiveness, Intubation and
Tracheostomy
Course FacultyRev. 9/13/11 FINAL
2:30 PM Break
2:45 PM Discussion of how to Quantify Airway Responsiveness
Wayne Mitzner, Ph.D., Johns Hopkins Bloomberg School of Public Health
3:00 PM Concurrent Rotating Labs: (cont’d)
Lung Mechanics and Airway Responsiveness, Intubation and
Tracheostomy
Faculty
4:30 PM Lab Clean Up
4:45 PM Transportation to Highseas
6:00 Dinner, Highseas Dining Room
7:00 – 8:30 “Roundtable Discussion”
Highseas Living Room
Wednesday, September 21 - Assessment of Pulmonary Pathology
8:00 AM Introduction to the Light Microscope and Optimizing Image
Acquisition
Doug Taatjes, Ph.D., The University of Vermont
8:45 AM Pathology of Lung Disease
Teresa Southard, D.V.M., Ph.D., Cornell University
9:40 AM Break
10:00 AM Harvest & Fixation Techniques
Wayne Mitzner, Ph.D., Johns Hopkins Bloomberg School of Public Health
10:30 AM Normal Mouse Cytology
James J. Lee, Ph.D., Mayo Clinic Arizona
11:30 AM Lunch, Highseas Dining Room
12:30 PM Transportation to GRB Training Lab
12:45 PM Concurrent Rotating Labs:
Lung Harvest & Fixation; Sampling Techniques: Blood, Lavage;
Cytospin & Cover Slipping
Course FacultyRev. 9/13/11 FINAL
2:45 PM Break
3:00 PM Mouse Lung Pathology Rounds
Course Faculty
3:45 PM Mouse Lung/Blood Cytology
Course Faculty
4:15 PM Lab Clean Up
4:30 PM Transportation to Highseas
6:00 PM Lobster Dinner at Highseas
Thursday, September 22 - Quantitative Lung Imaging and Pathology
8:00 AM Considerations for Image Presentation and Imaging Ethics
Douglas Taatjes, Ph.D., The University of Vermont
9:00 AM Introduction to Morphometry/Stereology and Sampling
Matthias Ochs, M.D. , Hannover Medical School
10:30 AM Break
11:00 AM Basic Measures: Volume, Number, Surface and Length
Matthias Ochs, M.D. , Hannover Medical School
12:00 PM Lunch, Highseas Dining Room
Free Afternoon
6:00 Dinner and Summary Discussion, Highseas Dining Room
Friday, September 23
Departures
Please vacate your room at Highseas by 10:00 a.m.
If your flight departs later than this check out time, you are very welcome to stay at Highseas during the day until
departure and make use of the common areas of the house; however, we must ask that you remove your belongings
from your room by 10:00 a.m.

19 Sep - 24 Sep 2012

Bar Harbor
United States of America
meeting website