Workshop on Cryopreservation of Mouse Germplasm

The cryopreservation course is offered to teach methods in cryopreservation for research strains of mice. Several methods of cryopreservation are now available and because no single method is adequate for all the various strains of mice being developed, a variety of methods are taught. The course is designed primarily as a "hands-on" laboratory program in which participants will learn techniques for the cryopreservation of cleavage-stage embryos, spermatozoa and ovaries .

In addition, general principles of cryobiology, development of inventory databases for individual programs and adaptation of long-term storage systems and cryogenic equipment for different situations will be discussed. Please note: the new sperm cryopreservation procedure recently announced by The Jackson Laboratory will be taught in this workshop.
+ show speakers and program
Rev. 2/17/12
Workshop on Cryopreservation of Mouse Germplasm
June 17 – 21, 2012
The Jackson Laboratory in Bar Harbor, Maine.
Schedule
This schedule is subject to change.
For more information please contact Nancy Place, nancy.place@jax.org
Sunday, June 17, 2012– Travel Day
Monday, June 18, 2012
7:30am – Van Shuttle from Quimby House – 109 Cottage Street to The Jackson Laboratory
7:40am - Van Shuttle from Bar Harbor Village Green, Main Street (by the clock) to The Jackson
Laboratory
7:45-8:30a.m. Breakfast and Registration at The Jackson Laboratory
8:45-9:00a.m. Course Overview and Introductions (lecture)
Robert Taft, Ph.D., The Jackson Laboratory
9:00-10:00a.m. Cryopreservation Strategies (lecture)
Robert Taft, Ph.D., The Jackson Laboratory
10:00-10:15a.m. Break
10:15-11:30a.m. Mouse Reproductive Biology (lecture)
Robert Taft, Ph.D., The Jackson Laboratory
11:30-12:00p.m. Sperm Cryo (lecture)
Robert Taft, Ph.D., The Jackson Laboratory
12:00 – 12:45 Lunch at Roscoe’s
12:45-1:45p.m. Biomethods (NOTE: If you attended the Biomethods session during the Workshop
on Embryo Transfer, you do not need to attend this session)
Kristin Cough, The Jackson Laboratory
1:45 – 3:30 Sperm Cryo (practical)
Jane Farley, The Jackson Laboratory
3:30-5:45 Basic Cryobiology (lecture)
Stanley Leibo, Ph.D., University of New Orleans/Audubon Nature Institute
5:45 pm Shuttle from Lab to Quimby House/Bar Harbor Village GreenRev. 2/17/12
Tuesday, June 19, 2012
7:00a.m. Van Shuttle from Quimby House – 109 Cottage Street to The Jackson Laboratory
7:10am - Van Shuttle from Bar Harbor Village Green, Main Street (by the clock) to The Jackson
Laboratory
7:15am Breakfast at Roscoe’s
8:00-8:30a.m. Introduction to In Vitro Fertilization (lecture)
Robert Taft, Ph.D., The Jackson Laboratory
8.30-10.30a.m. IVF Lab (practical)
Jane Farley, The Jackson Laboratory
10.45 -11.00 a.m. Break
11:00-12:00p.m. IVF-Implementation and Troubleshooting (discussion)
Robert Taft, Ph.D., The Jackson Laboratory
12:00-12.45 p.m. Lunch at Roscoe’s
12.45-1.45 p.m. IVF Lab (practical)
Jane Farley, The Jackson Laboratory

1.45-2.45p.m. Data Management (lecture)
Robert Taft, Ph.D., The Jackson Laboratory
2.45-4.45p.m. Basic Cryobiology (lecture)
Stanley Leibo, Ph.D., University of New Orleans/Audubon Nature Institute
4.45-5:15p.m. Daily Wrap Up
5:15pm Shuttle from Lab to Quimby House/Bar Harbor Village Green
Wednesday, June 20, 2012
7:00a.m. - Van Shuttle from Quimby House – 109 Cottage Street to The Jackson Laboratory
7:10am - Van Shuttle from Bar Harbor Village Green, Main Street (by the clock) to The Jackson
Laboratory
7:15am Breakfast at Roscoe’s
8:00-10.00.am IVF Lab (practical)
Jane Farley, The Jackson Laboratory
10.00-10.15.am Break
10.15 -11.15.m. Introduction to Slow-Rate Freezing (lecture)
Stanley Leibo, Ph.D., University of New Orleans/Audubon Nature Institute Rev. 2/17/12
11:15-12:00 p.m. Lunch at Roscoe’s
12:00 – 1.00 p.m. IVF Lab (practical)
Jane Farley, The Jackson Laboratory
1:00 – 3.00 pm. Slow-Rate Freezing (practical)
Sian Clements, The Jackson Laboratory
3:00-3.10 p.m. Break
3:00 – 5.00 p.m. Slow-Rate Freezing (practical)
Sian Clements, The Jackson Laboratory
5:00pm Shuttle from lab to Quimby House/Bar Harbor Village Green
6:00pm Dinner for Workshop Participants and Instructors
Location To Be Announced
Thursday, June 21, 2012
7:00a.m. -Van Shuttle from Quimby House – 109 Cottage Street to The Jackson Laboratory
7:10am - Van Shuttle from Bar Harbor Village Green, Main Street (by the clock) to The Jackson
Laboratory
7:15am Breakfast at Roscoe’s
7:45 – 8:15 IVF Lab (practical)
Jane Farley, The Jackson Laboratory
8:15 - 8:45a.m Repository Operations- Quality Control (lecture)
Robert Taft, Ph.D., The Jackson Laboratory
8:45 - 9:30 a.m. Repository Operations- Implementation (lecture)
Robert Taft, Ph.D., The Jackson Laboratory
9:30 – 9:40 a.m. Break
9:40 – 10:30 a.m Introduction to Embryo Vitrification (theory)
Stanley Leibo, Ph.D., University of New Orleans/Audubon Nature Institute
10:30-12:00 a.m Vitrification Lab (practical)
Sian Clements, The Jackson Laboratory
12:00- 1.00 p.m. Lunch at Roscoe’s
1.00 - 2.30pm. Discussion
3:00 pm Shuttle from lab to Quimby House/Bar Harbor Village Green
End of Workshop

18 Jun - 21 Jun 2012

Bar Harbor
United States of America
meeting website