Lipid Mass Spectrometry and Lipidomics

ABOUT THE PRACTICAL COURSE

This course is to introduce the students to the latest technologies in mass spectrometry based lipidomics and to provide them with practical knowledge to apply these methods in their own research. The topics cover a broad range of lipid species including, phospholipids and sphingolipids, sterols, steroids and bile acids, eicosanoids and neutral lipids.



By attending this course the students will learn:

1) How to extract different classes of lipids from biological samples (cells, tissue, fluids) and sample preparation for mass spectrometry analysis. Practical issues such as how to avoid contaminant lipids bleaching from laboratory wares will be discussed.

2) Principles of lipid mass spectrometry, including various scanning modes e.g. negative- or positive-ion scans as different classes of lipids will preferentially ionise in one polarity rather than the other; tailored scanning methods such as precursor-ion scan, neutral-loss scan, etc for individual classes of lipid.

3) Experimental design, such as shotgun lipidomics, LC-MS, GC-MS and lipid imaging using MALDI-MS

4) Interpreting MS spectra, signature fragment ions of each class of lipids

5) Lipids quantification using mass spectrometry

6) Large scale data analysis using bioinformatics tools.
Useful web resources such as http://www.lipidmaps.org or http://sterolanalysis.org.uk/ .


+ show speakers and program
William


Griffiths


Swansea University


UK




Xianlin


Han


Sanford-Burnham Medical Research Institute


US




Albert


Koulman


MRC Human Nutrition Research


UK




Harald


Köfeler


Medical University of Graz


AT




Alwena


Morgan


Swansea University


UK




Robert


Murphy


University of Colorado


US




Valerie


O'Donnell


Cardiff University


UK




Tony


Postle


University of Southampton


UK




Andrej


Shevchenko


The Max Planck Institute of Molecular Cell Biology and Genetics


DE




Cedric


Shackleton


Children's Hospital Oakland Research Institute


US




Yuqin


Wang


Swansea University


UK


19 Aug - 24 Aug 2012
Swansea
United Kingdom
meeting website