The structural characterization of macromolecular complexes

This EMBO practical course aims to teach the strategic use of biochemical and biophysical techniques to facilitate structural studies of macromolecular complexes. The primary focus is on how to produce, purify and characterize multi-subunit protein and protein/nucleic acid complexes for structural analysis.
Topics include:
expression in bacteria, insect and mammalian cells;
affinity methods to purify protein components or entire complexes;
biochemical and biophysical characterization of complexes;
bioinformatic analysis of interaction networks;
structural analysis by crystallography, cryo-electron microscopy, NMR, and small angle X-ray/neutron scattering.
Emphasis will be placed on:
the latest advances in isolating complexes directly from endogenous sources and in reconstituting complexes from individually purified components;
techniques for assessing sample quality (homogeneity, stoichiometry, monodispersity);
combining structure determination methods to obtain a multi-resolution view of a given complex
The course is primarily aimed at Ph.D students and post-docs engaged in challenging projects concerning the structural biology of macromolecular complexes.
+ show speakers and program

SUNDAY, 1 JUNE

19:00 – 21:00
Welcome drink and buffet dinner



MONDAY, 2 JUNE

09:00 – 09:45
Christophe Romier/ Co-expression in E.coli

09:45 – 10:30
Imre Berger / Complex expression in Baculovirus

10:30 – 11:00
Coffee

11:00 – 11:45
Radu Aricescu / Mammalian cell expression

11:45 – 12:30
Song Tan / Production of macromolecular complexes

12:30 – 13:30
Lunch

13:30 – 15:30
Laboratory Practical I

15:30 – 16:00
Coffee

16:00 – 18:00
Laboratory Practical II



TUEDAY, 3 JUNE

09:00 – 09:45
Vladimir Rybin / Overview of Biophysical assays

09:45 – 10:30
Kiyoshi Nagai / Structural analysis of the spliceosome

10:30 – 11:00
Coffee

11:00 – 11:45
Montserrat Soler Lópex / Yeast 2-hybrid methods

11:45 – 12:30
Bertrand Séraphin / Identifying and Purifying complexes

12:30 – 13:30
Lunch

13:30 – 15:30
Laboratory Practical III

15:30 – 16:00
Poster Clips

16:00 – 18:00
Poster session 1



WEDNESDAY, 4 JUNE

09:00 – 09:45
Anne-Claude Gavin / Large-scale studies of complexes

09:45 – 10:30
Song Tan / Structural studies of a protein/DNA complex

10:30 – 11:00
Coffee

11:00 – 11:45
Julia Richardson / Biophysical & biochemical analysis of a DNA transposase

11:45 – 12:30
Tobias Madl / NMR of macromolecular complexes

12:30 – 13:30
Lunch

13:30 – 15:30
Laboratory Practical IV

15:30 – 16:00
Coffee

16:00 – 18:00
Poster session 2



THURSDAY, 5 JUNE

09:00 – 09:45
Helen Saibil / EM single particle analysis

09:45 – 10:30
John Briggs / Electron tomography

10:30 – 11:00
Coffee

11:00 – 11:45
Sriram Subramaniam / EM studies of the HIV-1 glycoprotein

11:45 – 12:30
Carlos Fernández Tornero / Structural analysis of RNA polymerases

12:30 – 13:30
Lunch

13:30 – 15:30
Laboratory Practical V

15:30 – 16:00
Coffee

16:00 – 18:00
Laboratory Practical VI



FRIDAY, 6 JUNE

09:00 – 09:45
Dmitri Svergun / Small-angle scattering analysis of complexes

09:45 – 10:30
Gregory Giannone / Super-resolution microscopy

10:30 – 11:00
Coffee

11:00 – 11:45
Alexandre Bonvin / Computational approaches to study complexes

11:45 – 12:30
Jason Chin / Chemical Biology to probe interactions

12:30 – 13:30
Lunch

13:30 – 15:30
Laboratory Practical VII

15:30 – 16:00
Coffee

16:00 – 18:00
Laboratory Practical VIII



SATURDAY, 7 JUNE

09:00 – 09:45
Darren Hart / Library methods for protein expression

09:45 – 10:30
Christiane Schaffitzel / CryoEM studies of ribosomal complexes

10:30 – 11:00
Coffee

11:00 – 11:45
Malene Ringjobing / NMR of intrinsically disordered proteins

11:45 – 12:30
Elisabetta Boeri Erba / Native mass spectrometry of complexes

2 Jun - 7 Jul 2014
Grenoble
France
meeting website