Workshop on Techniques in Modeling Human Cancer in Mice

Cancer is a genetically complex and biologically heterogeneous group of disorders. It has become increasingly clear that the laboratory mouse, the best genetically defined experimental model organism for humans, presents a major opportunity for rapid advancement in understanding the genetic basis and underlying biology of cancer.

The overall goal for our course, "Workshop on Techniques in Modeling Human Cancer in Mice', is to train a small group of young scientists (predoctoral, postdoctoral trainees, new investigators) in the use of genetically defined laboratory mice as genetic tools for asking questions about gene function and the role of genetics in the biology of cancer. A major goal of this intensive hands-on workshop is to provide students an experience with a variety of manipulations using commonly used mouse models that can be adapted for their own unique models. Students completing the course acquire a practical knowledge of how to characterize and analyze specific mouse cancer models, and skills in a variety of cancer model manipulations so that students will have a broad skill set to apply techniques and manipulations to their own mouse cancer modeling research. The practical laboratory sessions will use a variety of normal and cancer models, and experimental manipulations using breast, prostate, brain, lung, leukemia/lymphoma and colorectal cancer models. The techniques learned are broadly applicable to many other cancer types, as well. Didactic lectures will reflect current knowledge of modeling cancer in a variety of organ sites and will emphasize common themes in cancer research and modeling. Lectures will be given in the morning, followed by intensive laboratory sessions whereby students will gain hands on experience in the manipulation and analysis of representative mouse models. Sessions will include fixed and live cell imaging techniques, cytometry, organ explants, surgical approaches to the implantation of tumors, and pathology.

The course takes place over a period of 7 days and is limited to 16 young investigators chosen for their outstanding research potential. The size of the class is kept deliberately small in order to achieve a desirable level of student-faculty interaction and to permit extensive laboratory training and practice for the students. The course is held annually at Highseas, The Jackson Laboratory’s residential oceanfront conference facility.
+ show speakers and program
The 2014 event schedule is being developed and will be posted as soon as possible. In the meantime, please view the 2013 event schedule for an idea of course content and structure.

Saturday, October 19, 2013

Location: Highseas Conference Center

4:30pm Registration and social hour

5:30pm Dinner

Location: Highseas Living Room

6:30pm Orientation
Jennifer Corrigan, M.S., The Jackson Laboratory

Introductions
Current and future challenges in cancer research
Carol Bult, Ph.D., The Jackson Laboratory
Bob Cormier, Ph.D., University of Minnesota – Duluth
Dusty Deming, M.D., University of Wisconsin
Richard Halberg, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham
Kevin Mills, Ph.D., The Jackson Laboratory
Amy Moser, Ph.D., University of Wisconsin
Katerina Politi, Ph.D.., Yale University
Lenny Shultz, Ph.D., The Jackson Laboratory
Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin
David Threadgill, Ph.D., Texas A & M University
Benjamin Titz, Ph.D., University of Wisconsin, - Madison
Kyuson Yun, Ph.D., The Jackson Laboratory

Sunday, October 20, 2013

Location: Highseas Conference Center

7:00am Breakfast

Location: Highseas Conference Center – 3rd floor conference room

8:30am Laboratory Mouse – History, genetics, tools and more
David Threadgill, Ph.D., Texas A&M University

9:30am Mouse strain nomenclature: What’s in a name?
Jennifer Corrigan, M.S., The Jackson Laboratory

10:30am Van transports participants to GRB 3rd Floor Training Laboratory

Location: GRB Training Laboratory

10:45am Biomethodology of the mouse - Handling, restraint, injections, and other
Jennifer Corrigan, M.S., The Jackson Laboratory

11:45pm Lunch – Roscoe’s

12:45pm Necropsy
Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin

1:45pm Preparation of organoid cultures
Bob Cormier, Ph.D., University of Minnesota – Duluth

6:00pm Van transports participants back to Highseas Conference Center

6:30pm Dinner – Highseas Conference Center

Location: Highseas Conference Center – 3rd floor conference

7:30pm Genetic screens with Sleeping Beauty
Bob Cormier, Ph.D., University of Minnesota – Duluth

Monday, October 21, 2013

Location: Highseas Conference Center

7:00am Breakfast

Location: Highseas Conference Center – 3rd floor conference

8:30am Models of Breast Cancer
Amy Moser, Ph.D., University of Wisconsin
9:30am Mammary metastasis models and use of cancer cell lines
Doug Hurst, Ph.D., University of Alabama at Birmingham

10:30am Van transports participants to GRB 3rd Floor Training Laboratory

Location: GRB Training Laboratory

10:45am Dissection of mammary glands, whole mount preparation, whole mount
analysis
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

12:45pm Lunch – Roscoe’s

1:45pm Mammary gland palpations, tumor and fat pad collection, lung collection,
lymph node collection
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

3:30pm Fat pad clearing for mammary cell transplantation, orthotopic injections
into the mammary fat pad
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

5:00pm Pathology slide review 1
Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin

6:00pm Van transports participants back to Highseas Conference Center

6:30pm Dinner – Highseas Conference Center

Tuesday, October 22, 2013

Location: Highseas Conference Center

7:00am Breakfast

Location: Highseas Conference Center – 3rd floor conference

8:30am Models of colon cancer
David Threadgill, Ph.D., Texas A&M University
Richard Halberg, Ph.D., University of Wisconsin

10:30am Van transports participants to GRB 3rd Floor Training Laboratory

Location: GRB Training Laboratory

10:45am Endoscopy techniques
Andrew Hillhouse, Ph.D., Texas A&M University

12:30pm Lunch – Roscoe’s

1:30pm Dissection and tumor quantification
Richard Halberg, Ph.D., University of Wisconsin
David Threadgill, Ph.D., Texas A&M University

4:30pm Pathology Slide review 2
Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin

5:30pm Van transports participants back to Highseas Conference Center

6:00pm Dinner – Highseas Conference Center

Location: Highseas Conference Center – 3rd floor conference


7:30pm Pathology lecture
Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin


Wednesday, October 23, 2013

6:00am Optional Hike – Precipice Trail, One of the steepest and most dramatic climbs in
Acadia, the hiking trail has many rungs and ladders along the trail’s length and is
approximately 1.6 mile/2.6 kilometers round trip providing you with a bird’s-eye
view of the coast

Location: Highseas Conference Center

7:00am Breakfast

Location: Highseas Conference Center – 3rd floor conference

9:30am Models of lung cancer
Katerina Politi, Ph.D.., Yale University

10:30am Van transports participants to GRB 3rd Floor Training Laboratory

Location: GRB Training Laboratory

10:45am Delivery of adenovirus to the colon and lung
Jamie Hadac, B.A., University of Wisconsin-Madison
Katerina Politi, Ph.D., Yale University

12:30pm Lunch – Roscoe’s

1:30pm Palpating for internal tumors, harvesting urogenital system, micro-
dissection of prostate, castration, ovarectomy, lung preparation
Katerina Politi, Ph.D., Yale University

3:30pm Kidney capsule implantation
Jennifer Corrigan, M.S., The Jackson Laboratory

5:30pm Van transports participants back to Highseas Conference Center

6:00pm Dinner

Location: Highseas Conference Center – 3rd floor conference

7:30pm Oncologist perspective of mouse models of human cancer
Dusty Deming, M.D., University of Wisconsin


Thursday, October 24, 2013

Location: Highseas Conference Center

7:00am Breakfast

Location: Highseas Conference Center – 3rd floor conference

8:30am Imaging
Benjamin Titz, Ph.D., University of Wisconsin - Madison

9:40am Van transports participants to Physiology Suite (FGB 44) **

IMPORTANT NOTE: The mouse ultrasound demo, the CT and Xenogen imaging lecture and the
microscopy tour, scheduled for 10:00 am-12:30pm on Thursday, October 24th, will take place
behind a barrier area. In order to participate in these sessions, individuals must not have
contact with rodents outside of The Jackson Laboratory for 5 days prior to this session.
Additionally, individuals must shower prior to coming to The Jackson Laboratory on this day,
wear laundered clothes and, as with all laboratory sessions, must wear socks, closed toe shoes
and have no exposed skin below the waist.

10:00am Rotating Sessions (Meet in the main lobby)
-Group 1 = Mouse Ultrasound Demo, CT and Xenogen Imaging
Jennifer Ryan, B.S. and James Denegre, Ph.D., The Jackson Laboratory
-Group 2 = Flow cytometry and microscopy
Ted Duffy and Mark Lessard, The Jackson Laboratory

11:10am Meet in the main lobby and switch groups

11:20am Rotating Sessions (Meet in the main lobby)
-Group 2 = Mouse Ultrasound Demo, CT and Xenogen Imaging
Jennifer Ryan, B.S. and James Denegre, Ph.D., The Jackson Laboratory
-Group 1 = Flow cytometry and microscopy
Ted Duffy and Mark Lessard, The Jackson Laboratory

12:30pm Lunch – Roscoe’s

1:30pm Leukemia/Lymphoma Techniques (Palpation of lymph node tumors, tumor
lymph node dissection, spleen dissection and splenocyte isolation, bone marrow
isolation, IP/retro-orbital/tail-vein injections and retro-orbital bleeding)
Kristin Lamont, Ph.D., and Jane Branca, The Jackson Laboratory

5:30pm Van transports participants back to Highseas Conference Center

6:00pm Lobster Dinner

Location: Highseas Conference Center – 3rd floor conference

7:30pm Imaging and monitoring tumor response to therapeutic intervention
Dusty Deming, M.D., University of Wisconsin

Friday, October 25, 2013

Location: Highseas Conference Center

7:00am Breakfast

Location: Highseas Conference Center – 3rd floor conference

8:30am Tipping the Balance: Targeting Genomic Instability for Lymphoma and
Leukemia Treatment
Kevin Mills, Ph.D., The Jackson Laboratory

10:00am Modeling brain cancer in mice
Kyuson Yun, Ph.D., The Jackson Laboratory

11:00am Humanized mice for cancer research
Lenny Shultz, Ph.D., The Jackson Laboratory

12:00pm Lunch – Highseas

1:00pm Mouse tumor biology (MTB) database
Carol Bult, Ph.D., The Jackson Laboratory

2:00pm Van transports participants to GRB 3rd Floor Training Laboratory

Location: GRB Training Laboratory

2:15pm Repeat or develop new techniques

5:30pm Van transports participants back to Highseas Conference Center

6:00pm Dinner

Location: Highseas Living Room

7:30pm Perspectives gained and needed


Saturday, October 26, 2013

Breakfast and departures

5 Sep - 12 Sep 2014
Bar Harbor
United States of America
meeting website