Workshop on the Cryopreservation of Mouse Germplasm

The cryopreservation course is offered to teach methods in cryopreservation for research strains of mice. Several methods of cryopreservation are now available and because no single method is adequate for all strains of mice being developed, several methods are taught. The course is designed primarily as a "hands-on" laboratory program in which participants will learn techniques for the cryopreservation of cleavage-stage embryos, spermatozoa and ovaries .

In addition, general principles of cryobiology, development of inventory databases for individual programs and adaptation of long-term storage systems and cryogenic equipment for different situations will be discussed. Please note: the new sperm cryopreservation procedure recently developed by The Jackson Laboratory will be taught in this workshop.

Applicants who do not have the pre-requisite embryo handling skills will be required to enroll in the Embryo Transfer Workshop immediately prior to this course.

In order to protect the health of the large mouse colonies at the Laboratory, certain regulations are in effect regarding prior contact with laboratory animals or pet rodents. Specifically, investigators cannot enter certain areas of the Laboratory if they have had any contact (handling or presence in an animal room) with laboratory animals, especially rodents, within the previous five days. Some workshop activities are held in these restricted areas. It is important that course participants not have any contact with laboratory animals after 11:59 p.m. on Friday, May 24th.
+ show speakers and program
Sunday, May 18, 2014

Travel Day - Arrivals in Bar Harbor


Monday, May 19, 2014

8:45-9:00 a.m. Course Overview and Introductions (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

9:00-10:00 a.m. Cryopreservation Strategies (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

10:00-10:15 a.m. Break

10:15-11:30 a.m. Mouse Reproductive Biology (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

11:30-12:30 Part I: Basic Cryobiology (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

12:30 – 1:30 p.m. Lunch at Roscoe’s

1:30-2:45 p.m. Part II: Basic Cryobiology (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

2:45 p.m. Shuttle for those that attended the “Biomethods” and “Liquid Nitrogen Safety, Dry
Shipper Demonstration and Introduction to JAX Biobank” sessions during the
Workshop on Embryo Transfer.

2:45-4:00 p.m. Liquid Nitrogen Safety, Dry Shipper Demonstration and Introduction to JAX
Biobank, Bld64, B115 (NOTE: If you attended this session during the Workshop
on Embryo Transfer, you do not need to attend this session.)
Kurt Christiansen, The Jackson Laboratory
4:00-5:00 p.m. Biomethods (NOTE: If you attended the Biomethods session during the
Workshop on Embryo Transfer, you do not need to attend this session)
Kristin Cough, The Jackson Laboratory



Tuesday, May 20, 2014

8:00-8:30 a.m. Introduction to In Vitro Fertilization (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

8.30-10.30 a.m. IVF Lab (practical)
Jane Farley, The Jackson Laboratory

10.30-11.00 a.m. Break

11:00-12:00 p.m. IVF-Implementation and Troubleshooting (discussion)
Robert Taft, Ph.D., The Jackson Laboratory

12:00-12.45 p.m. Lunch at Roscoe’s

12.45-1.45 p.m. IVF Lab (practical)
Jane Farley, The Jackson Laboratory

1.45-2.45 p.m. Data Management (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

2.45-4.45 p.m. Basic Cryobiology (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

4.45-5:15 p.m. Daily Wrap Up



Wednesday, May 21, 2014

8:00-10.00 a.m. IVF Lab (practical)
Jane Farley, The Jackson Laboratory

10.00-10.15 a.m. Break

10.15-11.15 a.m. Introduction to Slow-Rate Freezing (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

11:15-12:00 p.m. Lunch at Roscoe’s

12:00–1.00 p.m. IVF Lab (practical)
Jane Farley, The Jackson Laboratory

1:00–3.00 pm. Slow-Rate Freezing (practical)
Bree Roberts, The Jackson Laboratory

3:00-3.10 p.m. Break Rev. 8/30/13 JC

3:10–5.00 p.m. Slow-Rate Freezing (practical)
Bree Roberts, The Jackson Laboratory


6:00 p.m. Group Dinner



Thursday, May 22, 2014

7:45–8:30 IVF Lab (practical)
Jane Farley, The Jackson Laboratory

8:30–9:30 a.m Introduction to Embryo Vitrification (Lecture)
Robert Taft, Ph.D., The Jackson Laboratory

9:30-11:00 a.m Vitrification Lab (practical)
Bree Roberts, The Jackson Laboratory

11:00-12:00 p.m. Sperm Cryo (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

12:00-1.00 p.m. Lunch at Roscoe’s

1:00–3:00 p.m. Sperm Cryo (practical)
Jane Farley, The Jackson Laboratory

3:00-3:30 p.m. Break

3:30-4:00 p.m Repository Operations- Quality Control (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

4:00-4:45 p.m. Repository Operations- Implementation (lecture)
Robert Taft, Ph.D., The Jackson Laboratory

4:45-5:00 p.m. Wrap-up Discussion



Friday, May 23, 2014

Departures – Travel Day

18 May - 23 May 2014
Bar Harbor
United States of America
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