Cell Penetrating Peptides

The study of cell penetrating peptides (CPPs) now extends into a third exciting decade and it is certain that these mostly cationic sequences (e.g. TAT; GRKKRRQRRRPQ) have enormous potential in biomedicine. Thus, an extended range of inert CPPs has proven utility for the delivery of highly active cargoes (peptide, proteins, drugs and oligonucleotides) able to influence the expression and function of intracellular targets. Moreover, targeting sequences can more selectively direct CPPs to specific cell and tissue types and so improve both pharmacokinetic and pharmacodynamic profiles. More recent applications of CPPs in vivo, coupled with the development of bioactive CPPs (bioportides), provides further scope to employ CPPs as research tools, diagnostics and therapeutics.

In addition to lectures covering contemporary aspects of CPP design, synthesis and applications, four workshops will enable all delegates to consider their current and anticipated research activities and design CPPs that might be applied to support these. Thus, the conference will specifically provide a holistic framework to include modern methods of synthesis, CPP design (including targeting) and considerations of pharmacodynamics and pharmacokinetics that can be manipulated to achieve a desirable action of CPP-delivered bioactive cargoes either in vitro or in vivo.

Topics:

Design and synthesis of cell penetrating peptides (CPPs)
CPP-mediated siRNA and oligonucleotide delivery
Protein transduction and membrane translocation
Cell- and tissue-selective delivery of CPPs
CPP prediction
Bioactive CPPs
CPPs and in vivo applications
Recent developments in CPP technology
+ show speakers and program
Monday 7 October 2013

14:30 - 15:00
Introduction to the Course
John Howl / Sarah Jones (University of Wolverhampton, United Kingdom)
15:00 - 16:00
CPP Vectors to Bioportides
Sarah Jones (University of Wolverhampton, United Kingdom)
16:00 - 16:30 Coffee/tea break
16:30 - 17:30
Workshop - CPP Design for Specific Applications
The Team
19:30 Dinner
Design and Synthesis

Tuesday 8 October 2013
09:00 - 10:00 Round Table Discussion
10:00 - 11:00
CPP design and Intracellular Quantification with Mass
Fabienne Burlina (CNRS UMR Paris, France)
11:00 - 11:30 Coffee/tea break
11:30 - 12:30
Prediction of CPPs, CPPs as Pro-dugs, Intracellular
Mattias Hällbrink (Cepep 2, Stockholm, Sweden)
12:30 - 14:00 Lunch
14:00 - 15:00
Basic Principles of Peptide Synthesis
Peter White (NovaBiochem/Merck, United Kingdom)
15:00 - 15:30 Coffee/tea break
15:30 - 17:30
Workshop - Practical Methodologies
The Team
19:30 Dinner
Exploiting and Improving the Utility of CPPs

Wednesday 9 October 2013
09:00 - 10:00 Round Table Discussion
10:00 - 11:00
CPP Uptake Mechanisms: Endocytosis and Direct Membrane Translocation
Shiroh Futaki (Kyoto University, Japan)
11:00 - 11:30 Coffee/tea break
11:30 - 12:30
Biophysical Approaches to Identify Membrane and Intracellular Partners of CPPs
Sandrine Sagan (CNRS UMR, Paris, France)
12:30 - 14:00 Lunch
14:00 - 15:00
Biological Responses to Common CPPs, Fluorescence-based Determination of CPP uptake
Roland Brock (Radboud University Nijmegen, The Netherlands)
15:00 - 15:30 Coffee/tea break
15:30 - 17:30
Workshop - Refinement of CPP Design
The Team
19:30 Dinner
Applications of CPPs in Health and Disease

Thursday 10 October 2013
09:00 - 10:00 Round Table Discussion
10:00 - 11:00
PepFect Vectors, Delivery of siRNA and Oligonucleotides
Ülo Langel (University of Stockholm, Sweden)
11:00 - 11:30 Coffee/tea break
11:30 - 12:30
Targeted Deliver of CPPs and Phage Display Technologies
Pirjo Laakkonen (University of Helsinki, Finland)
12:30 - 14:00 Lunch
14:00 - 15:00
Utility of CPPs in vivo
Kaido Kurrikoff (University of Tartu, Estonia)
15:00 - 15:30 Coffee/tea break
15:30 - 18:30
Workshop - De novo CPP Applications
Delegate Presentations
19:30 Gala Dinner

7 Oct - 10 Oct 2013
London
United Kingdom
meeting website