Principles, parameters and applications of Polymerase Chain Reaction (PCR)

Aim of the course:
This short course aims at training the participant in the basic principles and applications of the Polymerase Chain Reaction (PCR) and real-time PCR. The program consists of a series lectures and experiments covering all basic PCR topics like efficiency, fidelity and specificity of the PCR reaction.

Theory:
Principles of DNA amplification technologies; The biochemical and physical aspects of the PCR
Principles, parameters, and design of (real-time) PCR primers
Principles and parameters of detection of PCR products
Principles of Real-time PCR; detection formats, assay formats
PCR contamination prevention

Practicals:
Real-time PCR dilution series and melting curve analysis:
Optimization and determination of the sensitivity of real-time PCR with SybrGreen
Optimization and determination of the sensitivity of real-time PCR with Hybridisation probes


+ show speakers and program
not specified

21 Oct - 21 Oct 2013
Breda
Netherlands
meeting website