Protein Interactions and Networks

This popular lab-based course provides training in the latest laboratory techniques and bioinformatics tools necessary to discover protein interactions and understand protein function.

For 2013, the course has been expanded to cover protein interactions by FRET microscopy and will again combine practical work with computer-based data analysis.

The practical programme will be complemented by specific lectures in topical areas as well as a panel of distinguished guest speakers, who will present the latest research in the field.
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Programme

Protein Complexes by Mass Spectrometry
-affinity purification using TAP tag approach
-protein identification by Mass Spectrometry.

Yeast Two-Hybrid
-rapid methods for making Bait and Prey clones
-using mating assays to investigate specific interactions
-library screening: faster ways to find new partners.

Binary Protein Interactions/BIAcore
-low affinity extracellular receptor-ligand interactions by AVEXIS
-biophysical protein interaction parameters (binding constants and kinetics).

Bioinformatics
-network and pathway visualisation
-protein interaction prediction and analysis
-data integration.

Protein Interactions by FRET microscopy
-sample preparation
-intensity-based FRET microscopy
-Fluorescence Lifetime Imaging (FLIM)-based FRET microscopy
-image/data analysis.

Course instructors:
Jyoti Choudhary (Wellcome Trust Sanger Institute, Hinxton, UK)
Chris Sanderson (University of Liverpool, UK)
Gavin Wright (Wellcome Trust Sanger Institute, Hinxton, UK)
Anton Enright (European Bioinformatics Institute, Hinxton, UK)
Fred S. Wouters (University of Göttingen, Germany)

8 Aug - 16 Aug 2013
Cambridge
United Kingdom
meeting website