Intravital Microscopy, Flow Cytometry and Cell Sorting

The aim of this EMBO Practical Course is to introduce state-of-the-art and cutting-edge technologies in the field of analytical and preparative cytometry and to introduce into multiplexed cytometric “cytome” assessment, including cell mass spectrometry.
Furthermore, we will provide an introduction into intravital sample preparation and microscopy technique.
Internationally renowned experts will give lectures and teach in hands-on practical sessions.
+ show speakers and program
SUNDAY, 7 JULY
15:00-17:00 Arrival
17:00-20:00 Welcome Reception with Participant Intro and Poster Session

MONDAY, 8 JULY

Lecture Session I
9:00-13:00 Flow Cytometry and Cell Sorting
13:00-14:00 Lunch break
Practical Block 1
14:00-18:00 Flow Cytometry: Set up, analysis and documentation

TUESDAY, 9 JULY
Practical Block 2
9:00-13:00 Image-based Flow Cytometry
13:00-14:00 Lunch break
Practical Block 3
14:00-18:00 Flow Cytometry: bioinformatics & data analysis
18:00-19:00 grand round Flow Cytometry
19:00-20:00 Troubleshooting: results, tips tricks and pitfalls

WEDNESDAY, 10 JULY

Lecture Session II
9:00-13:00 Intravital Microscopy
13:00-14:00 lunch break
Practical Block 4
14:00-18:00 Practical Microscopy in organotypical models & basic data analysis
18:00-19:00 grand round Cell Sorting
19:00-22:00 Boat trip and buffet

THURSDAY, 11 JULY

Practical Block 5
9:00-13:00 Imaging in living organisms & advanced data analysis
13:00-14:00 Lunch break
Practical Block 6
14:00-18:00 Mass-spectometry of cells
18:00-19:00 grand round Intravital Microscopy

FRIDAY, 12 JULY
Practical Block 7
9:00-13:00 magneto-fourescent isolation of rare cells
13:00-14:00 Lunch break
Practical Block 8
14:00-18:00 flourescent-activated cell sorting for RNA analysis & large particle sorting
18:00-19:00 final grand round course evaluation
19:00 Course Party and Farewell Reception

SATURDAY, 13 JULY
Departure


Speakers:
Mario Assenmacher, Miltenyi Biotec, Bergisch-Gladbach, Germany
Ryan Brinkman,Terry Fox Laboratory, Vancouver, Canada
Hyun-Dong Chang, DRFZ Berlin, Germany
Andrea Cossarizza, University of Modena, Italy
Enric Esplugues, DRFZ Berlin, Germany
Marc-Thilo Figge, University of Jena, Germany
Andreas Grützkau, DRFZ Berlin, Germany
Leo Hansmann, Stanford University School of Medicine, USA
Anja Hauser, DRFZ Berlin, Germany
Petra Hoffmann, University of Regensburg, Germany
Toralf Kaiser, DRFZ Berlin, Germany
Jenny Kirsch, DRFZ Berlin, Germany
Desiree Kunkel, BCRT, Charité - Universitätsmedizin Berlin, Germany
Holden Maecker, Stanford University School of Medicine, USA
Thorsten Mempel, Harvard Medical School, Charlestown, USA
Bob Murphy, Carnegie Mellon University, Pittsburgh, USA
Raluca Niesner, DRFZ Berlin, Germany
Garry Nolan, Stanford University School of Medicine, USA
Emily Park, BD Biosciences, San Jose, USA
Helena Radbruch, Charité - Universitätsmedizin Berlin, Germany
Diether Recktenwald, BD Biosciences, San Jose, USA
Chiara Romagnani, DRFZ Berlin, Germany
Alexander Scheffold, DRFZ Berlin, Germany
Jörg Schlegel, Merck-Millipore, Switzerland
Vincent Shankey, Beckman-Coulter Inc., Miami, USA
Heinrich Spiecker, LaVision Biotec, Bielefeld, Germany
Attila Tarnok, University of Leipzig, Germany
Andreas Thiel, BCRT, Charité - Universitätsmedizin Berlin, Germany

7 Jul - 13 Jul 2013
Berlin
Germany
meeting website