Electron Microscopy and Stereology in Cell Biology

The aim of this course is to teach cutting-edge EM techniques for Cell Biologists on a high theoretical and practical level. EM is technically one of the most demanding set of approaches to learn; many aspects can only reasonably be understood by watching a specialist. Despite the vast number of technical books available, there is clearly no substitute for hands-on training. Therefore the main emphasis in this course is put on the practical training.

In order to gain a deeper understanding of structure-function relationships in the cell, it is necessary to implement affinity cytochemistry at the EM level. Ultrastructural immunocytochemistry represents the most definite technique to identify specific cell structures in situ with sufficient spatial resolution. Recently, the use of these EM approaches has become less prominent. Likely reasons for this phenomenon are the increasing power of confocal and video light microscopy on the one hand, on the other hand the expense and working difficulties of EM techniques have effect also on this downtrend. However, only EM techniques can bring the information about antigen localization at the ultrastructural level, when is necessary to distinguish between the many interesting organelles whose functions are coupled (e.g. Golgi, ER).

EM approaches complement light microscopy by providing data unavailable through other methods. The course has a long tradition because it has been organized annually for about 30 years. During this period a stable teaching team has been formed who have great experience with teaching these techniques. It is composed of the world’s best specialists in processing of cells and tissues for EM in combination with immunogold labelling and quantitation via stereology. The teachers are able to teach someone with no previous experience in EM to a level where the students can not only establish the techniques in their home labs but also teach others.

In the course the students learn the best methods for preserving, visualizing, and quantifying antigens on cellular structures. They are encouraged to bring their own specimens and antibodies to the course.
+ show speakers and program
Preliminary Programme:

Day 1 - Sunday 16 June
Time
13:00-15:00 Instructors Get-together
15:00 - 15:30 Student Arrival
15:30 - 15:45 Welcome Address
15:45 - 19:00 Short talks by participants "7 min talks"
19:00 - 19:30 Short Lab tour with students
19:30 Get-together of students and teachers

Day 2 - Monday 17 June
09:00 - 09:45 Principles of transmission electron microscopy- Petr Chlanda, NIH
09:45 - 10:30 Principles of TEM Specimen preparation- John Lucocq, University of St Andrews Scotland
10:30 - 10:45 Coffee break
10:45 - 11:30 Approaches for plastic embedding and sectioning- Herb Hagler, UT Southwestern Medical Center
11:30 - 12:15 Introduction to Tokuyasu cryo-sectioning- Andreas Brech, Oslo University Hospital
12:15 - 13:00 Basics of Immunolabelling for TEM- Gareth Griffiths, University of Oslo
13:00 - 14:00 Lunch
14:00 - 18:00 Practicals
18:00 - 19:00 Dinner

Day 3 - Tuesday 18 June
09:15 - 10:00 Practical procedures in immunolabeling- Randi Olsen, University of Tromso
10:00 - 10:45 Colloidal gold- John Lucocq, University of St Andrews Scotland
10:45 - 11:00 Coffee break
11:00 - 11:45 Antibodies and the complexity of immunolabeling- Gareth Griffiths, University of Oslo
11:45 - 12:30 EM and applications in Biology- Rachel Mellwig, EMBL Heidelberg
12:30 - 13:30 Lunch
13:30 - 19:00 Practicals
19:00 - 21:00 Dinner

Day 4 - Wednesday 19 June
09:15 - 10:00 Chemical Fixation- Gareth Griffiths, University of Oslo
10:00 - 10:45 Vitrification for cryo EM- the basics- Petr Chlanda, NIH
10:45 - 11:00 Coffee break
11:00 - 11:45 Sampling, rapid freezing and freeze substitution- Heinz Schwarz, MPI for Developmental Biology
11:45 - 12:30 Adapting cryofixed material for immunolabelling- Heinz Schwarz, MPI for Developmental Biology
12:30 - 13:30 Lunch
13:30 - 19:00 Practicals
19:00 - 21:00 Dinner

Day 5 - Thursday 20 June
09:15 - 10:00 Introduction to Tomography- Petr Chlanda, NIH
10:00 - 10:45 Cryo EM and tomography- Marek Cyrklaff, Heidelberg University
10:45 - 11:00 Coffee break
11:00 - 12:30 New developments at the cutting edge- Helmut Gnaegi, Diatome Ltd
12:30 - 13:30 Lunch
13:30 - 19:00 Practicals
19:00 - 21:00 Dinner

Day 6 - Friday 21 June
09:15 - 10:00 Correlative light and electron microscopy- Heinz Schwarz and Yannick Schwab
10:00 - 10:45 Wide-scale morphomics using serial imaging- Yannick Schwab, EMBL Heidelberg
10:45 - 11:00 Coffee break
11:00 - 12:30 Practicals
12:30 - 13:30 Lunch
13:30 - 19:00 Practicals
19:00 - 21:00 Dinner

Day 7 - Saturday 22 June
09:15 - 10:00 tba
10:00 - 10:45 Basic approaches for quantifying gold labelling- John Lucocq, University of St Andrews Scotland
10:45 - 11:00 Coffee break
11:00 - 12:30 Exercises- Gold labelling quantification
12:30 - 13.30 Lunch
13:30 - 19:00 Practicals
19:00 - 21:00 Dinner

Day 8 - Sunday 23 June
09:15 - 13:00 Practicals all day
13:00 - 13:45 Lunch
13:45 - 19:00 Practicals and practical demonstrations
19:00 - 21:00 Dinner

Day 9 - Monday 24 June
09:15 - 10:00 tba
10:00 - 10:45 tba
10:45 - 11:00 Coffee break
11:00 - 12:30 Exercises
12:30 - 13:15 Lunch
13:15 - 18:30 Practical session
18:30 - 19:30 Dinner
19:30 Colloidal Gold from Theory to Practice- Jean Paul Goldmacher,

Day 10 - Tuesday 25 June
09:00 - 11:00 De-briefing talks
11:00 - 13:00 Practical session
13:00 - 13:45 Lunch
13:45 - 18:00 Practical session
18:00 - 19:30 Final discussion
19:30 Farewell Party

Day 11 - Wednesday 26 June
09:00 - 13:00 Practical session
13:00 - 13:45 Lunch
13:45 - 16:00 Practical session
16:00 End of the course

16 Jun - 26 Jun 2013
Heidelberg
Germany
meeting website