Exploiting Anomalous Scattering in Macromolecular Structure Determination

The objective of the practical course is the education of young structural biologists in the field of Macromolecular Crystallography. Course sessions will cover the theoretical and practical aspects of the determination of the three dimensional structure of a biomacromolecule, from optimal diffraction data collection to experimental phasing. Besides the well established methods for phase determination like MAD/SAD and Molecular Replacement, special emphasis will be given to new methods like sulphurSAD (SSAD) and RIP (radiation damage induced phasing) as well as to automated structure determination.
+ show speakers and program
MONDAY, 10 JUNE 2013
08:15
Registration in the ESRF entrance hall
Venue: ESRF auditorium
08:45-09:00
Welcome by Bauke Dijkstra, Director of Research of the ESRF, France
09:00-10:00
G. Leonard ESRF Grenoble, France
Introduction to anomalous scattering for phasing
10:00-10:15
Coffee break
10.15-11:00
S.McSweeney ESRF Grenoble, France
Introduction to Radiation Damage
11:00-12:00
A. Popov ESRF Grenoble, France
Data collection strategies accounting radiation damage
12:00-12:45
C. Müller-Dieckmann ESRF Grenoble, France
Introduction to ESRF Structural Biology Beamlines
12:45-14:00
Lunch at the ESRF/ILL restaurant
Venue: Beamlines and ESRF auditorium
14:00-18:00
Beamline practicals on BM14, ID23-1, ID23-2 & ID29
How to collect the best possible data
18:30-19:15
A. Bracher Max Planck Institute for Biochemistry Martinsried, Germany
Highlight #1
19:15
Dinner at the ESRF/ILL restaurant

TUESDAY, 11 JUNE 2013
Venue: ESRF auditorium
09:00-10:00
H. Powell MRC-LMB Cambridge, UK
Integration of diffraction images
10:00-11:00
W. Kabsch Max Planck Institute Heidelberg, Germany
Working principles of data processing with XDS
11:00-11:15
Coffee break
11.15-12:15
K. Diederichs University of Kostanz, Germany
Scaling and data quality
12:15-14:00
Lunch at the ESRF/ILL restaurant
14:00-15:00
H. Powell MRC-LMB Cambridge, UK
Mosflm and iMosflm - improved processing through a better interface
15:00-16:00
W. Kabsch Max Planck Institute Heidelberg, Germany
Data processing with XDS
16:00-16:15
Coffee break
16:15-17:15
I. Uson IBM Barcelona, Spain
Substructure determination
17:15-18:15
A. Thompson Soleil, France
Long Wavelength Phasing
18:15-19:00
A. Goldman University of Helsinki, Finland
Highlight #2
19:00-20:30
Poster session with ‘apéritif dinatoire’ at the ILL

WEDNESDAY, 12 JUNE 2013
Venue: ESRF auditorium
09:00-09:30
R.Giordano PSI Villigen, Switzerland
Multicrystal data collection
09:30-10:15
T. Terwilliger Los Alamos National Lab., USA
Density modification
10:15-10:45
Coffee break
10:45-11:30
I. Uson IBM Barcelona, Spain
Anomalous phasing with shelx c/d/e and hkl2map
11:30-12:15
C.Vonhrein Global Phasing Ltd Cambridge, UK
Automatic and non-automatic structure solution with SHARP/autoSHARP
12:15-14:00
Lunch at the ESRF/ILL restaurant
14:00-14:45
T. Terwilliger Los Alamos National Lab., USA
Automation of structure determination with Phenix
14:45-15:15
M. Nanao EMBL, Grenoble
Recent development in Radiation Damage Induced Phasing
15:15-16:15
Raj Pannu
Automatic protein structure solution from limited X-ray data
16:15-16:30
Coffee break
16.30-17:15
A. Mc Coy University of Cambridge, UK
Experimental phasing with phaser
17:15-18:15
C.Vonhrein Global Phasing Ltd Cambridge, UK
SHARP/autoSHARP
18:15-19:15
T. Terwilliger Los Alamos National Lab., USA
Phenix
19:30
Dinner at the ESRF/ILL restaurant

THURSDAY, 13 JUNE 2013
Venues: Beamlines and MD-1-21 seminar room
09:00-12:30
Collection of own data and roaming tutorials
12:30-14:00
Lunch at the ESRF/ILL restaurant
14:00-19:00
Collection of own data and roaming tutorials
19:00
Dinner at the ESRF/ILL restaurant

FRIDAY, 14 JUNE 2013
Venue: MD-1-21 seminar room
09:00-12:30
Roaming tutorials on own data
12:30-14:00
Lunch at the ESRF/ILL restaurant
14:00-16:00
Roaming tutorials on own data
Venue: ESRF auditorium
16:00-17:00
Presentations on own data (5' each)
17:00
Concluding remarks

10 Jun - 14 Jun 2013
Grenoble
France
meeting website