Small angle neutron and X-ray scattering from proteins in solution

This EMBO practical course will cover the use of small angle scattering (SAS) of both neutrons and X-rays for the determination of the structures of biological macromolecules. Particular attention will be paid on this course to sample preparation and the analysis and interpretation of SAS data in a biological context. The aim of this course is to enable the participants to maximise the information gained from the SAS technique in their future experiments.
In particular the course will focus on:
Small angle scattering theory
Sample requirements for a successful small angle scattering experiment
Data analysis, model building and interpretation
Isotope labelling strategies for small angle neutron scattering
Analysing small angle scattering in conjunction with data obtained from other techniques (NMR, AUC, EM, SEC-MALLS)

+ show speakers and program
PROGRAMME:
MONDAY, 6 MAY
Session I Small angle scattering: basic theory
09:00 - 09:10 Welcome
09:10 - 09:45 Introduction: What information can be obtained from SAS? Giuseppe Zaccai
09:45 - 10:30 Theory I: Basic information such as Rg, I(0), I(s or Q), absolute scale Frank Gabel
10:30 - 11:00 Coffee Break
Session II Practical considerations arising from small angle scattering theory
11:00 - 11:45 Theory II: Overview of different modelling techniques, complementarities and differences of X-rays and neutrons. Complementarities to NMR Frank Gabel
11:45 - 12:15 Contrast variation: use of natural contrast and labelling Giuseppe Zaccai
12:15 - 12:45 Panel discussion Frank Gabel, Giuseppe Zaccai
12:45 - 14:00 Lunch
Session III Data processing
14:00 - 14:45 Data reduction: 1D curves buffer subtraction, merging different concentrations Alexey Kikhney
14:45 - 15:30 Primary data analysis: Calculating model-independent parameters Alexey Kikhney
15:30 - 16:00 Coffee break
16:00 - 16:45 Ab initio modeling lecture: recovering 3D shapes from 1D data Daniel Franke
16:45 - 17:15 Ab initio modeling guided tutorial: demonstration of how to use DAMMIF Daniel Franke
17:15 - 18:30 Clip session: Short presentations of participants introducing themselves, their research and their reasons for attending
TUESDAY, 7 MAY
Session I Experimental practices
09:00 - 09:45 Sample characterisation: Quality control and sample handling prior to data collection Marc Jamin
09:45 - 10:30 Instrumentation and standard procedures: Overview of different instruments for small angle scattering and data collection procedures Adam Round, Anne Martel
10:30 - 11:00 Coffee Break
Session II Data analysis
11:00 - 11:45 Low and high resolution structure validation (CRYSOL/CRYSON/EM2DAM) and binary complex refinement Alexander Shkumatov
11:45 - 12:15 Guided tutorial: Use of structure validation tools Alexander Shkumatov
12:15 - 12:45 Burning questions: answering questions arising from the practical sessions
12:45 - 14:00 Lunch
Session III Advanced data analysis
14:00 - 14:45 Rigid body modelling lecture: Manual and automated refinement and addition of missing loops Maxim Petoukhov
14:45 - 15:30 Rigid body modelling guided tutorial: How to use MASSHA, SASREF and BUNCH Maxim Petoukhov
15:30 - 16:00 Coffee break
16:00 - 19:00 Poster session
WEDNESDAY, 8 MAY Data collection at SANS and SAXS beamlines at ILL and ESRF
4 groups of 5 students each (A, B, C and D)
08:30 - 10:30 Group A: Neutron experiment at ILL, Group B: X-ray experiment at ESRF, Groups C and D data analysis
10:30 - 11:00 Coffee break
11:00 - 13:00 Group C: Neutron experiment at ILL, Group D: X-ray experiment at ESRF, Groups A and B data analysis
13:00 - 14:00 Lunch
14:00 - 16:00 Group B: Neutron experiment at ILL, Group A: X-ray experiment at ESRF, Groups C and D data analysis
16:00 - 16:30 Coffee break
16:30 - 18:30 Group D: Neutron experiment at ILL, Group C: X-ray experiment at ESRF, Groups C and D data analysis
THURSDAY, 9 MAY
Session I Data analysis practical session
09:00 - 09:45 Tutorial: Independent tutorials, students working through data analysis examples in small groups
09:45 - 10:30 Burning questions: questions from students raised during the practical sessions and discussion so that all can adopt best practices with data analysis
10:30 - 11:00 Coffee Break
Session II Synergistic data analysis I
11:00 - 12:00 Structural information obtained by NMR Bernd Simon
12:00 - 13:00 Combined used of NMR and SAS for flexible systems Pau Bernado
13:00 - 14:00 Lunch
Session III Synergistic data analysis II
14:00 - 15:00 Combined use of neutrons and X-rays Lise Arleth
15:00 - 16:00 Structural information obtained from analytical ultracentrifugation Olwyn Byron
16:00 - 17:00 Structural information obtained from electron microscopy Guy Schoehn
17:00 - 19:00 Burning questions: questions from students raised during the practical sessions and discussion so that all can adopt best practices with data analysis
19:00 - 21:00 Course dinner
FRIDAY, 10 MAY
Session I Advanced strategies
09:00 - 09:45 Complex systems and mixtures forming fibrils Bente Vestergaard/Annette Eva Langkilde
09:45 - 10:30 Labeling strategies for small angle neutron scattering Michael Haertlein
10:30 - 11:00 Coffee Break
Session II Tutorials
11:00 - 12:45 Independent tutorials, students working through data analysis examples in small groups
12:45 - 14:00

Lunch
Wrap-up and keynote lecture
Session III
14:00 - 15:30 Student presentations and discussion: details of experiments and data analysis done during course: what were the most important lessions learned on the course?
15:30 - 16:00 Coffee break
16:00 - 17:00 Keynote lecture: Future of SAS; new instruments and opportunities for structural biologists Dmitri Svergun
17:00 - 19:00 Closing remarks and feedback from participants

6 May - 10 May 2013
Grenoble
France
meeting website