Frontiers and Techniques in Plant Science

This course provides an intensive overview of topics in plant physiology, biochemistry and development, focusing on genomic, analytical, computational and other high throughput approaches to understanding plant biology. It emphasizes recent results from model organisms including Arabidopsis, maize and tomato as well as a variety of other plants and provides an introduction to current methods used in basic and applied plant biology. It is designed for scientists with some experience in molecular techniques or in plant biology who wish to work with plants using the latest molecular, genomic, and computational technologies. The course consists of a vigorous lecture series, a hands-on laboratory, and informal discussions. The instructors and a stellar group of invited speakers, acknowledged leaders in their fields, will present up-to-the-moment research on a wide range of topics in plant research.

These seminars will include plant evolution, morphology and anatomy; various topics in plant development (including development of meristems, gametophytes, and roots); light perception and photomorphogenesis; cell wall biosynthesis and biofuels, function and perception of hormones; small RNAs; biotic and abiotic interactions; and applications addressing current agronomic problems. Lectures describing bioinformatics tools available to the plant community and the resources provided by plant genome projects are also included. Speakers will provide expert overviews of their fields, followed by in-depth discussions of their own work. The laboratory sessions will provide exposure to cutting edge techniques currently used in plant research. These include studies of plant development and genome evolution, transient gene expression, applications of fluorescent proteins, automated phenotyping, analysis of polysomal mRNA, analysis of global gene expression data (microarray and short-read sequencing), construction of genetic networks, and metabolome analysis. Students will be introduced to leading computational environments and programs including R, Bioconductor, and ImageJ. The course also includes several short workshops on important themes in plant research.

Lab & Lectures:
Introduction to Plant Structure
Phylogenetics
Sequence Analysis & Phylogeny Reconstruction
Light Regulation of Plant Development
Statistical Methods
Analysis of Microarray Data
Circadian Rhythms
Monitoring Gene Expression in Live Plants with Firefly Luciferase
Plant Kinematics
Phenotyping Using Machine Vision
Shoot Meristem Development
Fluorescence, Confocal and Scanning EM Imaging
Quantitative Genetics
Quantitative Trait Mapping Using Arabidopsis thaliana as a Model System
Plastids
In-planta Transient Expression in a C4 Model Grass
Root Development
Inferring Gene Networks
Secondary Metabolism
Analysis of Plant Volatiles
Proteomics
Plant Stress Responses
Immunoprecipitation of Polysomes from Transgenic Plants

Lectures:
Vegetative Development
Evolution of Plant Development
MicroRNA Regulation
Plant/virus interactions
Maize Genetics
Plant/Rhizobial Interactions
Gametophyte Development
Short Read Sequencing
Phyllotaxy
Cell walls and biofuels
Cytokinin Signaling
Modification of Plant Genomes
Genetics of Speciation
Genome Evolution and Speciation
Regulated Protein Degradation and Plant Signaling
Bioactive Plant Metabolites
Plant Systems Biology
Plant/Pathogen Interactions
Plant/Pest Interactions

Workshops:
Introduction to Genetics
Community resources
Evaluation of gas chromatograph/mass spectrometric data
Introduction to microscopy
Quantitative real-time PCR
Using Matlab for image analysis
+ show speakers and program
Instructors:
Stacey Harmer, University of California, Davis
Robert Last, Michigan State University
Shin Han Shiu, Michigan State University
Julin Maloof, University of California, Davis

28 May - 18 Jun 2013
Cold Spring Harbor
United States of America
meeting website